Preparation and In Vivo Evaluation of Indomethacin Loaded True Nanoemulsions
نویسندگان
چکیده
Indomethacin, a potent nonsteroidal anti-inflammatory drug, has been used in the treatment of various kinds of pains, inflammation and arthritis. However, oral administration of indomethacin produces serious gastrointestinal adverse effects. Therefore the aim of the present investigation was to evaluate the anti-inflammatory effects, skin irritation, activation energy and histopathology of indomethacin from transdermally applied true nanoemulsion. The anti-inflammatory effects of true nanoemulsions were compared with marketed Indobene(Â) gel on carrageenan-induced paw edema in rats. Skin irritation tests were performed on Wistar rats for 14 days. The % inhibition value after 12 h application was significant for optimized formulation F6 (83) as compared to marketed Indobene(Â) gel (P<0.01). Results of skin irritation test indicated that developed true nanoemulsion is safe for human use. The significant decrease in activation energy (1.396 kcal/mol) for indomethacin across rat skin indicated that the stratum corneum lipid bilayers were significantly disrupted (P<0.05). From these results it was concluded that the developed nanoemulsion have great potential for transdermal application of indomethacin.
منابع مشابه
Magnetic Nanoemulsions: Comparison between Nanoemulsions Formed by Ultrasonication and by Spontaneous Emulsification
Nanoemulsions are particularly suitable as a platform in the development of delivery systems. The type of nanoemulsion with a higher stability will offer an advantage in the preparation of a delivery system for lipophilic drugs. Nanoemulsions can be fabricated by different processing methods, which are usually categorized as either high- or low-energy methods. In this study, a comparison betwee...
متن کاملIn vivo evaluation of physiological efficacy of insulin-loaded nanoliposomes prepared for oral delivery
Introduction: Oral delivery is the most favorable route for insulin administration. The aim of this study was to generate new chitosan coated insulin nanoliposomes and then to assess the physiological efficacy of these nanoliposomes after oral administration in diabetic rabbits. Methods: Nanoliposomes with negative surface charge encapsulating insulin were prepared by reverse phase evaporat...
متن کاملBrinzolamide-Loaded Nanoemulsions: In vitro Release Evaluation
The aim of this investigation was to design and develop nanoemulsions (NEs) as novel ophthalmic delivery systems for brinzolamide (BZD). Phase behavior of quaternary systems composed of triacetin and CapryolTM 90 (selected oils, screened through the solubility studies), various surfactants (namely, Cremophor RH 40, Brij 35, Labrasol and tyloxapol), Transcutol P (as co-surfactant) an...
متن کاملPreparation, optimization and toxicity evaluation of (SPION-PLGA) ±PEG nanoparticles loaded with Gemcitabine as a multifunctional nanoparticle for therapeutic and diagnostic applications
The aim of this study was to develop a novel multifunctional nanoparticle, which encapsulates SPION and Gemcitabine in PLGA±PEG to form multifunctional drug delivery system. For this aim, super paramagnetic iron oxide nanoparticles (SPIONs) were synthesized and encapsulated simultaneously with Gemcitabine (Gem) in PLGA±PEG copolymers via W/O/W double emulsification method. Optimum size and enca...
متن کاملPreparation, optimization and toxicity evaluation of (SPION-PLGA) ±PEG nanoparticles loaded with Gemcitabine as a multifunctional nanoparticle for therapeutic and diagnostic applications
The aim of this study was to develop a novel multifunctional nanoparticle, which encapsulates SPION and Gemcitabine in PLGA±PEG to form multifunctional drug delivery system. For this aim, super paramagnetic iron oxide nanoparticles (SPIONs) were synthesized and encapsulated simultaneously with Gemcitabine (Gem) in PLGA±PEG copolymers via W/O/W double emulsification method. Optimum size and enca...
متن کامل